LC*GC

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COLUMNS

• COLUMN WATCH
  New Chromatography Columns and Accessories for 2018
  David S. Bell
  Our annual review of new liquid chromatography columns and accessories, including columns for reversed-phase, HILIC, chiral, SEC, GPC, ion-exchange, and SFC separations of small and large molecules.
  
• LC TROUBLESHOOTING
  Peak Purity in Liquid Chromatography, Part II: Potential of Curve Resolution Techniques
  Daniel W. Cook, Sarah C. Rutan, C.J. Venkatramani, and Dwight R. Stoll
  In part II of this series, we explore how curve resolution methods advance purity assessments and illustrate one of the most popular techniques, which adds a powerful tool to the chromatographer’s arsenal.
  
• PERSPECTIVES IN MODERN HPLC
  New HPLC Systems and Related Products Introduced in 2017–2018: A Brief Review
  Michael W. Dong
  An overview and assessment of the latest HPLC and mass spectrometry instruments, modules, chromatography data systems, and related software.
  
• FOCUS ON ENVIRONMENTAL ANALYSIS
  Best Practices for Analyzing Pesticides and Their Metabolites in Environmental Samples
  Laura Bush
  James Stry of FMC Agricultural Solutions talks about best practices for developing robust environmental analysis methods, including simplifying sample preparation and dealing with matrix effects.
  
• THE ESSENTIALS
  Making HILIC Work for You—Column Selection
  Ligands capable of undergoing electrostatic interactions can add an extra dimension to separations of ionizable compounds.
  

PEER-REVIEWED ARTICLE

• Data Integrity in the GxP Chromatography Laboratory, Part II: Setting up a Chromatograph and Acquiring Data
  Mark E. Newton and R.D. McDowall
  Part II of this series on practical perspectives on data integrity focuses on instrument setup, system suitability test samples, and data acquisition.
  

DEPARTMENTS

• Peaks of Interest
• Products & Resources
• Ad Index

COLUMNS

• SAMPLE PREP PERSPECTIVES
  Recent Advances in Solid-Phase Microextraction, Part I: New Tricks for an Old Dog
  Douglas E. Raynie
  A look at recent advances in SPME, such as increasing the sorbent surface area available for extraction, accommodating direct analysis by mass spectrometry, and sorbent overcoating to resist fouling by sugars and lipids
  
• LC TROUBLESHOOTING
  Eluent Preparation for Hydrophilic Interaction Liquid Chromatography, Part II: pH, Buffers, and Gradient Elution
  Dwight R. Stoll and Claudia Seidl
  Certain details related to mobile-phase preparation that can be assumed in reversed-phase LC separations can be much more consequential in HILIC.
  
• GC CONNECTIONS
  A Compendium of GC Detection, Past and Present
  John V. Hinshaw
  Gas chromatography makes use of a wide variety of detection methods, which really shine when deployed properly. Here’s guidance for how to choose the right one for your analysis.
  
• THE ESSENTIALS
  Optimizing SEC for Biologics Analysis
  Parameters such as pore size, column dimensions, temperature, flow rate, and mobile phase are important to consider when developing SEC methods.
  PEER-REVIEWED ARTICLES

PEER-REVIEWED ARTICLES

• Scaling LC Methods Using Superficially Porous Particle Stationary Phases
  Gregory K. Webster and Mathew A. Gragg
  Because not all laboratories have UHPLC systems, reverse scaling of methods from UHPLC to HPLC is often needed, particularly in the pharmaceutical industry. The use of superficially porous particles can facilitate this process.
  
• Chromatography Fundamentals, Part I: Thermodynamics of Liquid Chromatography: The Distribution Coefficient
  Howard G. Barth
  This article, the first in a series on chromatography fundamentals, discusses the cornerstone of all chromatographic techniques—the distribution or equilibrium coefficient—and its influence on liquid chromatographic separations.
  SPECIAL FEATURE

SPECIAL FEATURE

• Highlights of HPLC 2017 Jeju
  Ronald E. Majors
  A summary of the findings presented at the HPLC 2017 Jeju conference, reviewing how they fit into overall liquid-phase separation trends and the outlook for the future of these technologies
  

DEPARTMENTS

• Peaks of Interest
• Chromatography Corner
• Products & Resources
• Calendar
• Short Courses
• Ad Index

COLUMNS

• COLUMN WATCH
  Understanding the Science Behind Packing High-Efficiency Columns and Capillaries: Facts, Fundamentals, Challenges, and Future Directions
  Fabrice Gritti and M. Farooq Wahab
  Despite the theoretical promise of reduced plate heights (h) < 1, most modern UHPLC columns can only deliver plate heights in the range from 1.4 to 2.5. However, improved packing procedures, a better understanding of the colloidal properties of particle suspensions, and the study of the rheological behavior of packed beds and the final bed structure should lead us to practical solutions that can double the column efficiencies.
  
• LC TROUBLESHOOTING
  Peak Purity in Liquid Chromatography, Part I: Basic Concepts, Commercial Software, and Limitations
  Sarah C. Rutan, C.J. Venkatramani, and Dwight R. Stoll
  Is that peak “pure”? How do I know if there might be something hiding under there? In part I of this series, we explore some of the concepts behind peak purity assessments, describe some tools that are used in commercially available software for these assessments, and highlight some of the limitations of these tools using real-world examples.
  
• THE ESSENTIALS
  How to Avoid Problems in LC–MS
  Five tips to help you get the most out of your LC–MS instruments and methods
  

FEATURES

• The 2018 LCGC Awards
  Megan L’Heureux
  We explore the careers and achievements of the winners of LCGC’s 11th annual awards: Ronald E. Majors and Zachary S. Breitbach.
  
• Pittcon 2018: The LCGC Awards Symposium Preview
  In advance of Pittcon 2018, leading scientists—Ronald Majors, Richard Henry, John W. Dolan, Zachary S. Breitbach, and Daniel W. Armstrong—who will be speaking at the LCGC awards symposium give us a preview of their talks.
  

PEER-REVIEWED ARTICLE

• Accuracy Validation of Size-Exclusion Chromatography
  Howard G. Barth
  Here, a procedure is described for preparing polydisperse polymer standards to validate the accuracy of any SEC method for aqueous or organic mobile phases. This approach can be used for all calibration procedures with the exception of online light scattering and viscometric detection.
  

DEPARTMENTS

• Peaks of Interest
• Products & Resources
• Ad Index

COLUMNS

• SAMPLE PREP PERSPECTIVES
  Analyte Derivatization as an Important Tool for Sample Preparation
  Douglas E. Raynie
  There is a resurgence of interest in chemical derivatization schemes in separation science, particularly in sample preparation. Here’s why.
• LC TROUBLESHOOTING
  Eluent Preparation for Hydrophilic Interaction Liquid Chromatography, Part I: Solvent Volumes and Buffer Counterions
  Charles A. Lucy, Caley B. Craven, Claudia Seidl, and Dwight R. Stoll
  Paying attention to the details of mobile-phase preparation can have a big impact on the reproducibility of HILIC separations.
• FOCUS ON BIOPHARMACEUTICAL ANALYSIS
  Charge-Variant Profiling of Biopharmaceuticals
  Jared R. Auclair, Anurag Rathore, and Ira Krull
  A look at techniques for charge-variant analysis of monoclonal antibodies and the question of whether pH gradients are really better than salt gradients
• GC CONNECTIONS
  Tips and Tricks for New Gas Chromatographers
  Nicholas H. Snow
  Three main points summarize the best ways to be successful in GC: capillary GC is clean GC; practice, practice, practice; and be a student of GC.
• THE ESSENTIALS
  How It Works: Ion-Exchange SPE
  The fundamental principles of ion-exchange solid-phase extraction

PEER-REVIEWED ARTICLES

• Data Integrity in the GxP Chromatography Laboratory, Part I: Sampling and Sample Preparation
  Mark E. Newton and R.D. McDowall
  Part I of this series on practical perspectives of data integrity focuses on sample management, transport, and preparation.
• Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry for the Characterization of Polymeric Materials
  Peter Kusch
  HS-SPME-GC–MS is a valuable technique for identifying volatile organic compounds, additives, and degradation products in industrial rubber, car labeling reflection foil, and bone cement materials.

DEPARTMENTS

• Peaks of Interest
• Products & Resources
• Ad Index